Search results for "Most probable number"
showing 4 items of 4 documents
Use of functional genes to quantify denitrifiers in the environment.
2006
During the last decade, application of molecular methods using cultivation-independent approaches has provided new insights into the composition and structure of denitrifying communities in various environments. However, little is known about their abundance, and quantification is still performed using cultivation-based approaches, which are not only biased by the inability to cultivate of many micro-organisms but also fastidious and time-consuming. Two types of cultivation-independent approaches have recently been developed to quantify denitrifiers. The first type, which is based on the hybridization technique, comprises the use of Southern hybridization and DNA arrays. The second type, ba…
Microbial analysis of raw cows' milk used for cheese-making: influence of storage treatments on microbial composition and other technological traits
2010
Raw milk used to produce Grana cheese was subjected to several treatment regimes, including varying temperatures and storage times. Milk from morning and evening milking were transferred to a dairy factory sepa- rately (double delivery) or together (single delivery), after storage at the farm for 12 h; in the former case, milk was stored at 12 or 8°C, whereas, in the latter, it was kept at ambient temperature or 18°C. Values of pH of the vat milk were lower for milk samples kept at room temperature, while other physico-chemical parameters and rheological characteristics tested did not show significant differ- ences linked to the different storage temperatures of milk used for ‘‘Grana Trenti…
Assessment of the microbiological quality and wash treatments of lettuce served in University restaurants
2000
Abstract One hundred and forty-four samples of lettuce from 16 University restaurants were analyzed. The mesophilic aerobic counts of all samples ranged from 3.01 to 7.81 log 10 CFU g −1 . Results of total coliforms ranged from 3.38 log 10 most probable number (MPN) g −1 . Of the lettuce samples, 25.7% harbored Escherichia coli , 22.9% Staphylococcus aureus and 84% group D streptococci. Similarly, 10.4% of the samples harbored Aeromonas hydrophila , 2.8% Pseudomonas aeruginosa , and coliforms such as 14.6% Citrobacter freundii , 8.3% Klebsiella pneumoniae , 4.2% Enterobacter cloacae and 1.4% Providencia spp. Salmonella , Shigella and E. coli O157:H7 were not detected. When sodium hypochlori…
Méthode simple pour évaluer le potentiel endomycorhizogène d’un inoculum
2010
National audience; Les champignons mycorhizogènes à arbuscules (MA), forment une association mutualiste avec la plupart des plantes terrestres. Ils favorisent leur croissance et leur résistance à de nombreux stress biotiques et abiotiques. Leur utilisation en agriculture permet de limiter l’apport d’intrants chimiques. Pour gérer leur population il faut connaître leur abondance dans le sol, et donc évaluer le potentiel mycorhizogène du sol. Pour cela on utilise un test qui mesure le pouvoir de colonisation racinaire de ces symbiotes au niveau d’une plante piège. La méthode du « Most Probable Number » (MPN) est la plus utilisée. L’objectif de cette étude a été de comparer deux techniques d’é…